( lecture 1 ) Introduction to Molecular Genetics
Overview of molecular genetics00:00
Difference between DNA, RNA, and Proteins.00:00
Basics of DNA replication.00:00
Common DNA extraction methods.00:00
Perform DNA extraction using the phenol-chloroform method. ( Practical)00:00
Learn to calculate DNA concentration and purity ( practical)00:00
( Lecture 2 ) Gel Electrophoresis
What is gel electrophoresis and how does it work?00:00
Differences between DNA and RNA on a gel.00:00
Types of electrophoresis: • SDS-PAGE. • 2D Gel Electrophoresis.00:00
Prepare agarose gel for DNA separation ( practical)00:00
Load and run DNA samples ( practical)00:00
Visualize the gel under UV light (Practical)00:00
( Lecture 3 ) PCR (Polymerase Chain Reaction)
Molecular markers: RAPD, RFLP, SSR, ISSR, AFLP.00:00
Basics of Conventional PCR.00:00
Learn how to design primers for your experiment( practical)00:00
Prepare and set up a PCR reaction. ( Practical)00:00
( lecture 4) Data Analysis & Sequencing
Analyze PCR data and create DNA mapping dendrograms00:00
Introduction to DNA fingerprinting and sequencing.00:00
Create a DNA phylogenetic tree using sequencing data. ( Practical)00:00
Apply molecular docking techniques. (Practical)00:00